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HCV in serum, peripheral blood mononuclear cells and lymphocyte subpopulations in C-hepatitis patients.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Torres Martín Caballero Villalobos Olea |
| Copyright Year | 2000 |
| Abstract | To define the best marker for the follow-up and evaluation of HCV infections we determined anti-HCV antibodies, serum transaminases and HCV RNA in patients diagnosed with chronic hepatitis for C virus and treated with alpha-interferon. The presence/absence of HCV RNA was determined in serum, peripheral blood mononuclear cells (PBMC) and lymphocyte subpopulations. Samples were submitted to RT-PCR and subsequent nested PCR. Treatment with alpha-interferon induced a fall in the number of HCV RNA positive patients from an initial 88 to 25% at the end of the treatment. The withdrawal of treatment was associated with a significant increase in the number of HCV RNA positive patients (43% at the 12-month follow-up). In 61% of the patients the PCR analysis of the PBMC population detected the presence of HCV RNA. In 87% of cases the cell fraction identified as CD19 resulted positive in the PCR test and the viral genome was undetectable in PBMC subpopulations in only 13% of cases. In one third of the patients whose serum was negative for PCR the analysis demonstrated the presence of HCV RNA in PBMC. Conclusions: The disappearance of the viral genome in serum, a criterion of treatment response, is not necessarily followed by its disappearance in PBMC. The joint determination of HCV by PCR technique in serum and blood cells should be used as a particular instrument with each patient. |
| Starting Page | 141 |
| Ending Page | 151 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S1386-6346(99)00098-4 |
| PubMed reference number | 10936565 |
| Journal | Medline |
| Volume Number | 18 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://hera.ugr.es/doi/15015737.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S1386-6346%2899%2900098-4 |
| Journal | Hepatology research : the official journal of the Japan Society of Hepatology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
