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Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect
| Content Provider | Semantic Scholar |
|---|---|
| Author | Barron, Valerie Merghani, K. Shaw, Georgina Margaret Coleman, Christopher M. Hayes, J. S. Ansboro, Sharon Manian, Abdurrahman Malley, Grace O’ Connolly, Emma Nandakumar, Allyala Blitterswijk, Clemens Van Habibovic, Pamela Moroni, Lorenzo Shannon, Freeman Murphy, Josephine Mary Barry, Frank |
| Copyright Year | 2015 |
| Abstract | AbstractThe main objective of this study was to evaluate the effectiveness of a mesenchymal stem cell (MSC)-seeded polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT) scaffold for cartilage tissue repair in an osteochondral defect using a rabbit model. Material characterisation using scanning electron microscopy indicated that the scaffold had a 3D architecture characteristic of the additive manufacturing fabrication method, with a strut diameter of 296 ± 52 μm and a pore size of 512 ± 22 μm × 476 ± 25 μm × 180 ± 30 μm. In vitro optimisation revealed that the scaffold did not generate an adverse cell response, optimal cell loading conditions were achieved using 50 μg/ml fibronectin and a cell seeding density of 25 × 106 cells/ml and glycosaminoglycan (GAG) accumulation after 28 days culture in the presence of TGFβ3 indicated positive chondrogenesis. Cell-seeded scaffolds were implanted in osteochondral defects for 12 weeks, with cell-free scaffolds and empty defects employed as controls. On examination of toluidine blue staining for chondrogenesis and GAG accumulation, both the empty defect and the cell-seeded scaffold appeared to promote repair. However, the empty defect and the cell-free scaffold stained positive for collagen type I or fibrocartilage, while the cell-seeded scaffold stained positive for collagen type II indicative of hyaline cartilage and was statistically better than the cell-free scaffold in the blinded histological evaluation. In summary, MSCs in combination with a 3D PEOT/PBT scaffold created a reparative environment for cartilage repair. |
| Starting Page | 2069 |
| Ending Page | 2082 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s10439-015-1246-2 |
| PubMed reference number | 25589372 |
| Journal | Medline |
| Volume Number | 43 |
| Alternate Webpage(s) | https://aran.library.nuigalway.ie/bitstream/handle/10379/5868/ABME-D-14-00778-1_Submission.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://www.library.nuigalway.ie/bitstream/handle/10379/5868/ABME-D-14-00778-1_Submission.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://doi.org/10.1007/s10439-015-1246-2 |
| Journal | Annals of Biomedical Engineering |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
