NDLI: The Cotton WRKY Gene GhWRKY41 Positively Regulates Salt and Drought Stress Tolerance in Transgenic Nicotiana benthamiana

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The Cotton WRKY Gene GhWRKY41 Positively Regulates Salt and Drought Stress Tolerance in Transgenic Nicotiana benthamiana

Content Provider	Semantic Scholar
Author	Chu, Xiaoqian Wang, Chen Chen, Xiaobo Lu, Wenjing Wang, Xiuling Hao, Lili Guo, Xingqi
Copyright Year	2015
Abstract	WRKY transcription factors constitute a very large family of proteins in plants and participate in modulating plant biological processes, such as growth, development and stress responses. However, the exact roles of WRKY proteins are unclear, particularly in non-model plants. In this study, Gossypium hirsutum WRKY41 (GhWRKY41) was isolated and transformed into Nicotiana benthamiana. Our results showed that overexpression of GhWRKY41 enhanced the drought and salt stress tolerance of transgenic Nicotiana benthamiana. The transgenic plants exhibited lower malondialdehyde content and higher antioxidant enzyme activity, and the expression of antioxidant genes was upregulated in transgenic plants exposed to osmotic stress. A β-glucuronidase (GUS) staining assay showed that GhWRKY41 was highly expressed in the stomata when plants were exposed to osmotic stress, and plants overexpressing GhWRKY41 exhibited enhanced stomatal closure when they were exposed to osmotic stress. Taken together, our findings demonstrate that GhWRKY41 may enhance plant tolerance to stress by functioning as a positive regulator of stoma closure and by regulating reactive oxygen species (ROS) scavenging and the expression of antioxidant genes.
File Format	PDF HTM / HTML
DOI	10.1371/journal.pone.0143022
PubMed reference number	26562293
Journal	Medline
Volume Number	10
Alternate Webpage(s)	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0143022&type=printable
Alternate Webpage(s)	https://doi.org/10.1371/journal.pone.0143022
Journal	PloS one
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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