Loading...
Please wait, while we are loading the content...
Similar Documents
Dean Flow-coupled Inertial Focusing for Ultra-high- Throughput Particle Filtration
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ardabili, Sahar Gantelius, Jesper Kowalewski, Jacob M. Brismar, Hjalmar Russom, Aman |
| Copyright Year | 2010 |
| Abstract | Particle manipulation represents an important and fundamental step prior to counting, sorting and detecting bio- particles. In this study, we report dean-coupled inertial focusing of particles in flows through a single curve microchannel at extremely high channel Reynold numbers (~325). We found the lateral particle focusing position, xf to be fixed and largely independent of radius of curvature and whether particles are pre-focused (at equilibrium) entering the curvature or randomly distributed. Finally, using a single inlet, u-shaped, microchannel we demonstrate filtration of 10µm particles from 2 µm particles at throughputs several orders of magnitude higher than previously shown. Particle focusing is a necessary step for concentrating, detecting, sorting and focusing bio-particles. The enrichment of particles is an essential technique for sample pre-treatment for downstream bio-analyses. Microfluidics has the potential to overcome the shortcomings associated with large-scale equipment through reduction of analyte and reagent volumes as well as favorable scaling properties of several important instrument processes. Microfluidics is particularly amenable to gentle and high throughput cell handling. Very recent, inertial-induced forces developed in microchannels have been pro- posed by us and others as a promising approach for particle focusing, filtration and separation (1-4). In inertial microflui- dics, dominant inertial forces cause particles to move across streamlines and occupy equilibrium positions along the faces of microchannel walls (1). The addition of curvature reduces the focusing points by introducing a secondary cross- sectional flow field (Dean flow) characterized by the presence of two counter-rotating vortices located above and below the plane of symmetry of the channel. Using a spiral microchannel, we recently demonstrated for the first time the emer- gence of two focusing points along the height of the channel and hypothesized the balance between dominant lift forces (FL) and Dean forces (FD) as responsible for particle focusing (3-4). Although recent intense activity in the field, there is limited understanding on how the curvature affects the focusing of particles. In this study, using a u-shaped single curved microchannel, we systematically analysed the interaction of secondary forces with particles and show how randomly dis- tributed particles entering the curvature are focused at a fixed lateral position over a range of radius of curvature and channel aspect ratio. We apply the system for ultra-high throughput filtration applications. |
| Starting Page | 1586 |
| Ending Page | 1588 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/binaries/LOC/2010/PDFs/Papers/540_0803.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
