NDLI: Diosgenin inhibits hTERT gene expression in the A549 lung cancer cell line.

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Diosgenin inhibits hTERT gene expression in the A549 lung cancer cell line.

Content Provider	Semantic Scholar
Author	Mohammad, Rahmati Yamchi Somayyeh, Ghareghomi Gholamreza, Haddadchi Majid, Mobasseri Yousef, Rasmi
Copyright Year	2013
Abstract	BACKGROUND Diosgenin, a steroidal saponin from a therapeutic herb, fenugreek (Trigonellafoenum-graceum L.), has been recognized to have anticancer properties. Telomerase activity is not detected in typical healthy cells, while in cancer cell telomerase expression is reactivated, therefore providing a promising cancer therapeutic target. MATERIALS AND METHODS We studied the inhibitory effect of diosgenin on human telomerase reverse transcriptase gene (hTERT) expression which is critical for telomerase activity. MTT- assays and qRT-PCR analysis were conducted to assess cytotoxicity and hTERT gene expression inhibition effects, respectively. RESULTS MTT results showed that IC50 values for 24, 48 and 72h after treatment were 47, 44 and 43μM, respectively. Culturing cells with diosgenin treatment caused down-regulation of hTERT expression. DISCUSSION These results show that diosgenin inhibits telomerase activity by down-regulation of hTERT gene expression in the A549 lung cancer cell line.
Starting Page	1
Ending Page	2
Page Count	2
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/apocp/POCPA9/2013/v14n11/POCPA9_2013_v14n11_6945.pdf
PubMed reference number	24377630v1
Volume Number	14
Issue Number	11
Journal	Asian Pacific journal of cancer prevention : APJCP
Language	English
Access Restriction	Open
Subject Keyword	Carcinoma of lung Diosgenin Down-Regulation Estrogens, Non-Steroidal Gene Expression Inhibitory Concentration 50 Leukemia, B-Cell RNA-Directed DNA Polymerase Reverse Transcriptase Polymerase Chain Reaction Saponin TERT wt Allele cancer cell monooxyethylene trimethylolpropane tristearate telomerase activity
Content Type	Text
Resource Type	Article

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