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Investigation of the diaphorase reaction of ferredoxin-NADP+ reductase by electrochemical methods
| Content Provider | Semantic Scholar |
|---|---|
| Author | Madoz, Juan Roberto Rodriguez Fernández-Recio, Juan Gómez-Moreno, Carlos Fernández, Víctor Mendoza |
| Copyright Year | 1998 |
| Abstract | Abstract The enzyme ferredoxin-NADP+ reductase (FNR) can be used in amperometric enzyme electrodes for NADP+ detection. In order to evaluate the performance of such electrodes, a critical step is the comparison of the catalytic rate constants of immobilized FNR enzyme to the FNR in solution. In this work, electrochemical techniques such as cyclic voltammetry have been applied to investigate the catalysis of NADPH oxidation by ferredoxin-NADP+ reductase using one-electron redox mediators such as ferrocenemethanol. The activity of the enzyme in solution mediated by ferrocenemethanol is comparable to other one-electron redox mediators previously measured. |
| Starting Page | 179 |
| Ending Page | 183 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0302-4598(98)00175-5 |
| Alternate Webpage(s) | http://www.unizar.es/departamentos/bioquimica_biologia/investigacion/mmedina/Madoz1998.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S0302-4598%2898%2900175-5 |
| Volume Number | 47 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
