Targeted knockdown of death-associated protein kinase expression induces TRAIL-mediated apoptosis in human endometrial adenocarcinoma cells.

Content Provider	Semantic Scholar
Author	Tanaka, Tetsuji Yukawa, Kazunori
Copyright Year	2010
Abstract	Death-associated protein kinase (DAPK) is a serine/threonine kinase that participates in the modulation of apoptosis and tumor suppression. Our previous study revealed high levels of DAPK protein expression in differentiated endometrial adenocarcinoma cells. To clarify the role of DAPK in human endometrial adenocarcinomas, we down-regulated endogenous DAPK expression in HHUA cells, a well-differentiated endometrial adenocarcinoma cell line, using specific small-interfering RNAs (siRNAs). The suppression of endogenous DAPK expression triggered apoptosis in HHUA cells, as evidenced by an increase in the sub-G1 DNA content in flow cytometric analyses. The apoptosis induced by the DAPK siRNA transfections was caspase-dependent, as characterized by the activations of caspase-3, -8 and -9. RNase protection assays detected higher levels of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), DR4 and DR5 transcripts in the DAPK siRNA-transfected HHUA cells than in the control siRNA-transfected cells. Consistent with these findings, enzyme-linked immunosorbent assays revealed that the DAPK siRNA transfections significantly increased the secretion of TRAIL protein from the cells. Treatment with recombinant human TRAIL protein dose-dependently suppressed the cell viability of HHUA cells. The present findings reveal that down-regulation of endogenous DAPK expression in HHUA cells induces caspase-dependent apoptosis, possibly through increased TRAIL, DR4 and DR5 signaling, thereby suggesting that DAPK expression is essential for HHUA cell survival. Consequently, endogenous DAPK mRNA may represent a potential candidate for molecularly targeted anticancer therapies.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.spandidos-publications.com/ijo/37/1/203/download
PubMed reference number	20514412v1
Volume Number	37
Issue Number	1
Journal	International journal of oncology
Language	English
Access Restriction	Open
Subject Keyword	Biomarkers, Tumor Caspase-Dependent Apoptosis Cell Survival Cessation of life DAPK1 gene Down-Regulation Endometrial Carcinoma Endometrial adenocarcinoma Enzyme-Linked Immunosorbent Assay Ligands MARK2 gene Necrosis Neoplasms Non-Small Cell Lung Carcinoma Protein Kinases Protein-Serine-Threonine Kinases RNA, Small Interfering Recombinants TNF-related apoptosis-inducing ligand Threonine Transcript Transfection caspase-3 protein expression
Content Type	Text
Resource Type	Article