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Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex
| Content Provider | Semantic Scholar |
|---|---|
| Author | Benmansour, Saloua Bonnet, J. Protais, Philippe Costentin, Jean |
| Copyright Year | 1987 |
| Abstract | When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration. |
| Starting Page | 97 |
| Ending Page | 102 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0304-3940(87)90614-8 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0304394087906148 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0304394087906148?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 3601221 |
| Alternate Webpage(s) | https://doi.org/10.1016/0304-3940%2887%2990614-8 |
| Journal | Medline |
| Volume Number | 77 |
| Journal | Neuroscience Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
