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Characterization of Bacteria Producing Acyl Homoserine Lactone (AHL) Lactonase from Agricultural Lands
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fitriyah, Dina Wahyudi, Aris Bambang Tri Rusmana, Iman |
| Copyright Year | 2015 |
| Abstract | Gram negative phytophatogen bacteria perform quorum sensing by using N-acylhomoserine lactone signal to regulate the expression of virulence factor genes. One of strategy to inactivate quorum sensing by degrading the signal molecules using AHL lactonase encoded by aiiA gene. This study was conducted to characterize bacteria producing AHL lactonase from agriculture lands. Isolation of AHL degrading bacteria was done on 11 sampels of rhizosphere soils from Java, Indonesian agriculture lands. AHL degrading activities of bacterial isolates were tested using Chromobacterium violaceum as a bioindicator. AHL degrading bacterial isolates were indentified based on morphology and 16S rRNA gene. Verification of AHL lactonase existence was done by amplification of aiiA gene (using BTF and BTR primers). A total of 161 bacterial isolates were isolated from rhizosphere soils, six of them could degrade AHL. Analysis of 16S rRNA gene sequences showed that the isolates had 100% similarity with Serratia marcescens By2Root2 (BKS-1 isolate), Bacillus aquimaris JB306 (BKS-8), B. marisflavi GN28 (BGR-7), B. altitudinis NIOT-BARREN23 (CMS-4), B. aquimaris JP44SK28 (JBR1-3) and B. axarquiensis CHMS1B6 (JBR2-16), respectively. The aiiA gene amplification showed that all isolates were successfully amplified with PCR product of 750 bp. BLAST-X analysis of aiiA gene showed that AHL lactonase of BKS-1 was closely related with AHL lactonase B. cereus group, BKS-8 with AHL lactonase Bacillus sp. MBG09, BGR-7 with AHL lactonase Bacillus sp. MBG12, CMS-4 and JBR1-3 were closely related with AHL lactonase B. cereus, JBR2-16 with AHL lactonase B. firmus with 99%, 92%, 97%, 94%, 98%, and 99% of maximum identity, respectively. Phylogenetic analysis based on amino acid sequences of AHL lactonase (AiiA) showed that AHL lactonase from the six isolates had similarity with AHL lactonase of B. cereus, B. weihenstaphenensis, B. thuringiensis, B. subtilis and B. firmus, respectively. This finding indicated new information of quorum quenching bacteria that B. aquimaris, B. marisflavi, B. altitudinis, B. axarquiensis and S. marecescens had AHL lactonase encoded by aiiA gene and they might have potential application to degrade AHL of plant pathogenic bacteria. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.aensiweb.net/AENSIWEB/aeb/aeb/2015/May/140-148.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
