NDLI: Effects of low Light on Terpenoid Indole Alkaloid Accumulation and Related Biosynthetic Pathway Gene Expression in Leaves of Catharanthus roseus Seedlings

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Effects of low Light on Terpenoid Indole Alkaloid Accumulation and Related Biosynthetic Pathway Gene Expression in Leaves of Catharanthus roseus Seedlings

Content Provider	Semantic Scholar
Author	Liu, Ying Zha, Dongmei Zu, Yuangang Tang, Zhonghua Zhang, Zhonghua Jiang, Yang Shi, Dian-Yi
Copyright Year	2011
Abstract	Catharanthus roseus (L.) G. Don, an important medicinal plant, produces the powerful alkaloids vinblastine (VBL) and vincristine (VCR), which are known for their remarkable inhibitory activity against tumor cells. This paper is the result of a study on the effects of low light/UV light on the contents of chlorophyll and alkaloids, and on the expression of terpenoid indole alkaloids (TIAs) biosynthesis pathway genes in the leaves of aseptic C. roseus seedlings. A plastic film treatment first induced a slight increase and then a significant decrease (p＜0.05) in the total chlorophyll content, and a lower chlorophyll a/b ratio. The amounts of vindoline (VIN) and catharanthine (CAT) followed the identical pattern under plastic film but the content of VBL, generated by its precursors VIN and CAT, increased gradually until reaching a maximum (0.028 mg g^(-1)±0.0051) on the 15th day of treatment. The plastic film also induced the expression changes of TIA biosynthesis pathway genes. The further correlation analysis between alkaloid content and these genes transcriptional levels indicated that VIN accumulation was significantly correlated with the gene expression of desacetoxyvindoline-4-hydroxylase (D4h) and deacetylvindoline-4-O-acetyl transferase (Dat), and CAT accumulation was significantly correlated with strictosidine synthase (Str) gene expression (p＜0.05). Therefore, the low light treatment with plastic film accelerated the accumulation of VBL in the leaves of C. roseus seedlings, which may have practical significance in the production of VBL.
Starting Page	191
Ending Page	196
Page Count	6
File Format	PDF HTM / HTML
Volume Number	52
Alternate Webpage(s)	http://ejournal.sinica.edu.tw/bbas/content/2011/2/Bot522-08.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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