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Hydroxyl radical generation by polymorphonuclear leukocytes measured by electron spin resonance spectroscopy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rosen, Henry Klebanoff, Seymour J. |
| Copyright Year | 1979 |
| Abstract | Electron spin resonance spectroscopy using the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was employed to detect the formation of hydroxyl radicals (OH.) by phagocytosing polymorphonuclear leukocytes (PMN). An electron spin resonance signal with the identical g value and splitting characteristics of the DMPO/OH). adduct was detected on incubation of normal PMN with opsonized zymosan. Adduct formation was strongly inhibited by superoxide dismutase and by the OH. scavenger mannitol, but catalase had little or no effect. (DMPO/OH). was not formed by PMN from a patient with chronic granulomatous disease; in contrast, adduct formation by PMN which lack myeloperoxidase was greater than normal. These findings are discussed in relation to the formation of OH. by PMN. |
| Starting Page | 47 |
| Ending Page | 53 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://dm5migu4zj3pb.cloudfront.net/manuscripts/109000/109637/JCI79109637.pdf |
| PubMed reference number | 227939v1 |
| Volume Number | 64 |
| Issue Number | 6 |
| Journal | The Journal of clinical investigation |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CATALASE Chronic granulomatous disease Electron Spin Resonance Spectroscopy Gas Scavengers Hydroxyl Radical Mannitol Patients Superoxide Dismutase Superoxides Zymosan adduct neutrophil |
| Content Type | Text |
| Resource Type | Article |
