Effects of Aqueous Extract of Hibiscus sabdariffa L. (Roselle) Calyx on Bovine Sperm Membrane

Content Provider	Semantic Scholar
Author	Jamaludin, Mohamed Sabir Hanis, Muhd Balkis, Siti Mohamed, Jamaludin
Copyright Year	2012
Abstract	Sperm cryopreservation is a crucial in preserving sperm quality and studying male fertility defects. However, the process will cause several sperm abnormalities as it will produce excessive ROS that damage the sperm. As consequences, membrane cell disrupt, DNA of the sperm will damage and sperm motility diminished as wells as its viability deteriorated. Therefore, this study was conducted to determine the ability of aqueous extract of roselle calyx in protecting sperm freezing1thawing condition. UKMR12 type has been chosen because it contains high antioxidants compare to other variety of roselle. All the control and treated group are incubated at 37°C for six hours. Extraction of sperm membrane is done to get pure sperm membrane. Sperm membrane that was treated with aqueous extract of roselle calyx of 15 mg/ml, 25 mg/ml and 50 mg/ml showed a significant increased compared with control. Concentration of malondealdehyde (MDA) in sperm membrane treated with aqueous extract of roselle calyx concentration of 15 mg/ml, 25 mg/ml and 50 mg/ml are decreased compared to control. It showed less lipid peroxidation occurred in the membrane that was treated with aqueous extract of roselle calyx. The increasing and decreasing of protein and MDA were depending on the dose of the roselle concentration. There were no significant changes in the sperm morphology of the three treatment groups of the aqueous extract of roselle compared with control group. In conclusion, this study found that the aqueous extract of roselle calyx could reduce ROS production in sperm freezing1thawing process.
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Language	English
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Content Type	Text
Resource Type	Article