NDLI: Rapid differentiation of Prevotella intermedia and P. nigrescens by 16S rDNA PCR-RFLP.

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Rapid differentiation of Prevotella intermedia and P. nigrescens by 16S rDNA PCR-RFLP.

Content Provider	Semantic Scholar
Author	Milsom, Susan E. Sprague, Shelley Dymock, David Weightman, Andrew Wade, William G.
Copyright Year	1996
Abstract	Prevotella intermedia and the newly described P. nigrescens cannot be reliably distinguished by phenotypic tests. In this study, restriction endonuclease digestion of amplified 16S rDNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the type strains of P. intermedia and P. nigrescens and 43 fresh isolates identified as belonging to one of the two species. Whole-cell protein profiles were obtained by SDS-PAGE for comparative purposes. The type strains of P. intermedia and P. nigrescens were easily distinguished by 16S rDNA PCR-RFLP and the fresh isolates were assigned to either species on the basis of their restriction profiles. The identifications obtained were identical to those obtained by protein profiles. 16S rDNA PCR-RFLP is a rapid and reliable method for the differentiation of P. intermedia and P. nigrescens.
Starting Page	1
Ending Page	4
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jmm/44/1/medmicro-44-1-41.pdf?accname=guest&checksum=348BA95702EFC1F9546E7B54C72CBAEA&expires=1542594307&id=id
PubMed reference number	8544210v1
Volume Number	44
Issue Number	1
Journal	Journal of medical microbiology
Language	English
Access Restriction	Open
Subject Keyword	Cell Differentiation process DNA Restriction Enzymes PersonNameUse - assigned Polyacrylamide Gel Electrophoresis Prevotella intermedia Restriction fragment length polymorphism endonuclease
Content Type	Text
Resource Type	Article

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