Loading...
Please wait, while we are loading the content...
Similar Documents
Tissue engineering of the intervertebral disc with cultured annulus fibrosus cells using atelocollagen honeycombshaped scaffold with a membrane seal (ACHMS scaffold)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sato, Maho Kikuchi, Manami Ishihara, M. Asazuma, Takashi Kikuchi, Takatoshi Masuoka, Kazunori Hattori, Hidemi Fujikawa, Kyosuke |
| Copyright Year | 2006 |
| Abstract | The objective of the study was to investigate the regeneration of intervertebral discs after laser discectomy using tissue engineering procedures. Annulus fibrosus (AF) cells from the intervertebral discs of Japanese white rabbits were cultured in an atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS scaffold), to produce a high-density, three-dimensional culture for up to 3 weeks. Although the DNA content in the scaffold increased at a lower rate than that in the monolayer culture, expression of type ll collagen and glycosaminoglycan accumulation in the scaffold were at higher levels than in the monolayer. The AF cells that had been cultured in the scaffold for 7 days were allografted into the lacunae of intervertebral discs of recipients (40 rabbits, 14–16 weeks old; average weight, 3.2kg), whose nucleus pulposus (NP) had been vaporised with an ICG dye-enhanced laser. The allografted cultured AF cells survived and produced hyaline-like cartilage. Furthermore, the narrowing of the intervertebral disc space of the cell-containing scaffold insertion groups was significantly inhibited after 12 post-operative weeks. |
| Starting Page | 365 |
| Ending Page | 371 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF02348444 |
| PubMed reference number | 12803304 |
| Journal | Medline |
| Volume Number | 41 |
| Alternate Webpage(s) | http://cellsheet.med.u-tokai.ac.jp/pdf/2-12.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/BF02348444 |
| Journal | Medical and Biological Engineering and Computing |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
