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Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Jiquan Raper, Anna Sugita, Noriko Hingorani, Ravi Salio, Mariolina Palmowski, Michael J. Cerundolo, Vincenzo Crocker, Paul R. |
| Copyright Year | 2006 |
| Abstract | We describe the cloning and characterization of Siglec-H, a novel murine CD33-related siglec-like molecule with 2 immunoglobulin domains. Unlike other CD33-related siglecs, Siglec-H lacks tyrosine-based signaling motifs in its cytoplasmic tail. Although Siglec-H has the typical structural features required for sialic acid binding, no evidence for carbohydrate recognition was obtained. Specific monoclonal and polyclonal antibodies (Abs) were raised to Siglec-H and used to define its cellular expression pattern and functional properties. By flow cytometry, Siglec-H was expressed specifically on plasmacytoid dendritic cell (pDC) precursors in bone marrow, spleen, blood, and lymph nodes. Staining of tissue sections showed that Siglec-H was also expressed in a subset of marginal zone macrophages in the spleen and in medullary macrophages in lymph nodes. Using bone marrow-derived pDC precursors that express Siglec-H, addition of Abs did not influence cytokine production, either in the presence or absence of synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG). In comparison, Siglec-H functioned as an endocytic receptor and mediated efficient internalization of anti-Siglec-H Abs. By immunizing mice with ovalbumin-conjugated anti-Siglec-H Ab in the presence of CpG, we demonstrate generation of antigen-specific CD8 T cells in vivo. Targeting Siglec-H may therefore be a useful way of delivering antigens to pDC precursors for cross-presentation. |
| Starting Page | 89 |
| Ending Page | 98 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/107/9/3600.full.pdf?sso-checked=true |
| PubMed reference number | 16397130v1 |
| Volume Number | 107 |
| Issue Number | 9 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Antibodies Bone Marrow Carbohydrates Cross-Presentation Cytoplasmic Domain Cytosine Flow Cytometry Guanine Immunoglobulin Domains Immunostimulating conjugate (antigen) Leukemia, B-Cell N-Acetylneuraminic Acid Oligodeoxyribonucleotides Ovalbumin Plasmacytoid Dendritic Cells Sialic Acid Binding Immunoglobulin-like Lectins Spleen Tissue Tyrosine cytidylyl-3'-5'-guanosine cytokine production lymph nodes |
| Content Type | Text |
| Resource Type | Article |
