Effect of Eucalyptus citriodora extract on hsp 70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster ( hsp 70-lacZ )

Content Provider	Semantic Scholar
Author	Rahul, Smita Jyoti Naz, Falaq Shakya, Barkha Khan, Safiya Afzal, Mohammad Hasan, Yasir Siddique
Copyright Year	2012
Abstract	The effect of leaf extract of Eucalyptus citriodora was studied on hsp70 expression by using soluble O-nitrophenyl-β D-galactopyranoside (ONPG) assay. The tissue damage was evaluated by trypan blue exclusion assay. The extract was studied at 1, 2, 4, 6, 8, 10 and 12 μl/ml of food concentrations and third instar larvae were exposed to these concentrations for 24 and 48 hrs. The results showed a significant dose dependent increase in expression of hsp70. The tissue damage was not observed for the 24 hrs of exposure at all concentrations studied, very little damage was observed after 48hrs of exposure in salivary glands and malpighian tubules at 12μl/ml of food concentration of extract. The dose of 1 μl/ml of extract did not induced significantly, expression of hsp70 for 24 hrs of exposure, but expression was significantly higher as compared to control for 48 hrs of exposure at the same dose i.e. 1μl/ml.
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Language	English
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Content Type	Text
Resource Type	Article