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Vascular endothelial growth factor is bound in amniotic fluid and maternal serum.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Vuorela-Vepsäläinen, P. Alfthan, Henrik V. Orpana, Arto K. Alitalo, Kari Stenman, Ulf Hakan Halmesmäki, Erja |
| Copyright Year | 1999 |
| Abstract | To study vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) concentrations and their possible binders, serum from 22 non-pregnant and 55 pregnant women (15 at weeks 10-13; 40 at term), umbilical vein (n = 24) and artery (n = 13) and amniotic fluid (a pool of 50 at weeks 15-17; 11 at term) were assessed for VEGF and PlGF by an enzyme-linked immunosorbent assay. In amniotic fluid and maternal serum VEGF concentrations were <16 ng/ml and added VEGF was not recovered. VEGF was detected in serum from mothers post-partum (137 +/- 142 ng/l, mean +/- SD), umbilical artery (421 +/- 288 ng/l) and vein (502 +/- 339 ng/l) and non-pregnant controls (182 +/- 147 ng/l), and added VEGF was fully recovered. PlGF was detected in pregnancy serum (52 +/- 23 ng/l early pregnancy; 439 +/- 217 ng/l term pregnancy) and in amniotic fluid (early pregnancy 56 ng/l; term pregnancy 30 +/- 18 ng/l). PlGF was fully recovered in all samples. Gel filtration and isoelectric focusing revealed that in maternal serum and amniotic fluid [125I]VEGF was bound to a protein with an Mr of 400-700 kDa and an isoelectric point of approximately 8. This protein was not identical with alpha-2-macroglobulin (by an immunofluorometric assay), pregnancy zone protein or pregnancy associated plasma protein-A (by immunodiffusion). In conclusion, VEGF-binding activity is present in amniotic fluid and maternal blood. It disappears after delivery and is not detectable in fetal or non-pregnant serum. |
| Starting Page | 453 |
| Ending Page | 456 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://watermark.silverchair.com/141346.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAjMwggIvBgkqhkiG9w0BBwagggIgMIICHAIBADCCAhUGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMO15rqiwBqCzh2sCTAgEQgIIB5peR29JcAnxYMBLrjKBvMqEbdyUJ85pgjY_oJYZS6e4O5nyWLw4UQH2CrmhoU1CAKWLrgR0jTp5ski9l-W_vxnNraguGnVHouR6aQkqfcfTxA5XVQOT51tFD-QqPUz22R5srTgMcipg_iw9G-dxwbjiq5qfWqBmKBap8SF-kAX4Uj6pIqCPuW4jASJc818zdo3DI8LE2rtAzRdDRPo4c_5gvGXN3efmfCydidwVLUVgE83qTJkijcJy1UMT7dHQe7ww9tvhNCDPOPVVcRi5mYw2keEQ9hWC4DQSSI2FjcH6p_M68rX2YSbLKbQX0mypcxXHETZtPWyr9PMWnRf9AFHZ9ifa9qwf_QHt396t_mBV_IIH40vjfsYjmkEFWbG4LrNsMKn5MWGAzebpUSUbs7JzfYDwvTmqJFHCXDu_og6j41t_arrfbKKsEq_BFCDHWuX_rwH8hv0vV6ryRIrrqj5ohX-K5243g49rIDh3hGIHW0_w8lUoCfulPQbx6fwE2L6FLXDtFxtqHpCN9lgVeYanL7rmRPnP2KjdgMNfCcs0AVTogf186hxxo1YjnZyJXbkUyl9Q_8U6SUUJgPxEYv3b_dJ-yG5jKZ27zjTR1sxNIgffHnW6hKQWZLw9f3pwlhSPCEVVsUQ |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/humrep/14/5/10.1093_humrep_14.5.1346/1/141346.pdf?Expires=1492998528&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=bt0uf6L1o~yzrgftmrd6XMefn0WSwwVtcJtFm24glLwmPkhyDXomLicmhwI3SyhKFNJEPK~V0X47diIMzi~IVo-ZG1YdBrZkh4P0nsPvUvm9ll7XO93ieXNwKWHd1EnWRuJYOSK9QINge0Me~yQR8sCPyVX3n8exEf5ZLuXKM~sZ3zNT0ht1kZx~geavJMpD3~HwRCVCxleFCaFoTkF4WpQSyBG3Xj2BIK9b58G7P5MHFTj68eNmlEE6Gkbniz2pxkzK~FQ~8SCPvuIPrKSgz3mSKjUeXNTaBcl2ZLhyGsKQ6AfR0XFZH3cD6d8PFXFfHus0ZH9KxYlCY7ZqF5mLSA__ |
| PubMed reference number | 10325292v1 |
| Volume Number | 14 |
| Issue Number | 5 |
| Journal | Human reproduction |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Endothelial Growth Factors Enzyme-Linked Immunosorbent Assay Fluoroimmunoassay Immunodiffusion measurement Isoelectric Focusing Isoelectric Point Microgram per Liter Nanogram per Liter Partum Physiological Sexual Disorders Placental Growth Factor Staphylococcal Protein A Structure of umbilical artery Umbilical vein Umbilicus (Anatomy) VEGF protein, human alpha 2-Glucoproteins |
| Content Type | Text |
| Resource Type | Article |
