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Advances in Multi-focus Microscopy: Acquiring and Visualizing 3d Super-resolution Images
| Content Provider | Semantic Scholar |
|---|---|
| Author | Beheiry, Mohamed El Hajj, Bassam Chen, Jiji Dahan, Maxime |
| Copyright Year | 2013 |
| Abstract | Super-resolution microscopy beyond the conventional diffraction limit is an invaluable tool for understanding biological processes, making it possible to infer the morphology of molecular assemblies, examine the composition of intracellular complexes, and understand the dynamic behavior of single-molecules. An important challenge for studying biological specimen is the development of robust methods for accessing super-resolution information in 3D. Our recently demonstrated multi-focus microscopy (MFM) technique enables the acquisition of 3D stacks in a parallel and ultrasensitive manner, with a measurement depth of ~4 μm (exceeding astigmatism, double-helix, and biplane techniques) [1]. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://focusonmicroscopy.org/2013/PDF/130_ElBeheiry.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |