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Efeitos da terapia a laser associada ou não ao plasma rico em plaquetas na reparação do tecido muscular de ratos
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ozaki, Guilherme Akio Tamura |
| Copyright Year | 2015 |
| Abstract | Treatment of muscle injuries usually results in the interruption of sports practice, so studies aimed at anticipating the return to activity, with proper tissue repair become important. Therefore, this study intended to evaluate the effect of low level laser therapy (LLLT) associated or not with platelet-rich plasma (PRP) in the treatment of muscle injury. Forty two animals were used divided into six groups (n=7), Control (C), Acute Injury Control (CLA), Injury Control (CL), Injury LLLT (LLT), injury PRP (LP), and injury LLLT and PRP (LLtP). Muscle injury were induced by stretching the gastrocnemius muscle and the animals of groups LLtP and LP received the application of PRP immediately after the injury. The LLLT were applied daily for seven days. The animals were euthanized seven days after the injury, except the CLA group, which were euthanized 48 hours after injury. Histological slides of the gastrocnemius muscle were prepared without stained for Raman analyze and stained by Hematoxylin and eosin (HE), and Picrosirius witch are analyzed by fractal dimension (DF), analyzing the inflammatory infiltrated and collagen. Analysis of NADH and NAD were performed by Raman spectroscopy. The histological analyzes showed great destruction of the tissue, and the treatments were effective in promote the repair in the tissue, furthermore the LLtP group demonstrate better histological aspect than the treatments isolated, even in relation to the extension of the lesion as well as the regeneration process. The fractal analyze showed high DF values of the inflammatory process (HE) in the experimental groups, CLA and CL in relation to C (p = 0.000). The analysis of collagen in picrosirius stained slides showed high DF in CLA group compared to CL (p = 0.0001), with no statistically significant difference between the other groups. The ratio of NADH/NAD showed oxidative stress on injured groups (CLA and CL). In summary, the present study demonstrated that fractal analysis was able to identify changes from the inflammatory process in injured skeletal muscle tissue, by the analysis of slides stained with HE, and changes in the collagen, in slides stained with picrosirius. Furthermore the treatments were effective in repair the damage caused by muscle injury, and both the LLLt as the PRP and the combination of both were able to reduce the oxidative stress caused by injury, and modulate the collagen production. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://repositorio.unesp.br/bitstream/handle/11449/128107/000848921.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | http://www2.fct.unesp.br/pos/fisioterapia/dissertacao/15/guilherme_ozaki.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
