NDLI: Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity

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Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity

Content Provider	Semantic Scholar
Author	Huang, Yingying Mao, Qiqi Su, Jing Peng, Yi Liang, Wei Hu, Zixi Zhou, Sufang Lu, Xiaoling Zhao, Yongxiang
Copyright Year	2017
Abstract	To explore dendritic cells/tumor-derived endothelial cells (DC/EC) fusion cells are potent stimulators of T cells to impact tumor progression. ECs were isolated from mice hepatoma cell line (H22) Xenograft, and dendritic cells were isolated from bone marrow of BALB/c mice, then the isolated ECs were cultured and detected the endothelial surface expression of CD105 by flow cytometry. The endothelial characteristics of ECs were detected by tube formation assay and Dil-Ac-LDL uptake assay. After the fusion with polyethylene glycol (PEG), we used DCs, ECs, DCs mixed ECs as the control groups, DC/EC fusion cells as the experimental group, Secretion of IFN-α and IFN-γ was evaluated, T lymphocyte proliferation and cytotoxic T lymphocytes (CTL) were detected in vitro. In vivo, T lymphocyte induced by five groups was injected to detect the effect of tumor progression. Purified ECs (CD105+) took the function of endothelial cells, then successfully fused with DCs. The DC/EC fusion cells were functional in stimulating the proliferation of T cells, which produced IFN-α and IFN-γ. In vivo, T cells stimulated by DC/EC fusion cells effectively repressed tumor growth. The fusion cells, which was capable of stimulating T cells, is indispensable for antitumor immunity.
Starting Page	305
Ending Page	318
Page Count	14
File Format	PDF HTM / HTML
PubMed reference number	28436481
Alternate Webpage(s)	https://doi.org/10.1038/srep46544
DOI	10.1038/srep46544
Journal	Scientific reports
Volume Number	7
Language	English
Access Restriction	Open
Subject Keyword	Bone Marrow Cytotoxic T-Lymphocytes Deoxycytidine Endothelium Flow Cytometry In Vitro [Publication Type] Lymphocyte Count measurement Neoplasms Polyethylene Glycols Stimulation (motivation) T-Lymphocyte Tumor Progression childhood lymphocyte depletion Hodgkin's lymphoma
Content Type	Text
Resource Type	Article

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