Regulation of exoprotein gene expression by the Staphylococcus aureus cvfB gene.

Content Provider	Semantic Scholar
Author	Matsumoto, Yasuhiko Kaito, Chikara Morishita, Daisuke Kurokawa, Kenji Sekimizu, Kazuhisa
Copyright Year	2007
Abstract	We previously reported that the cvfB gene (SA1223) of Staphylococcus aureus is responsible for the virulence of this pathogenic bacterium. We show here that the cvfB gene regulates exoprotein gene expression. In a cvfB gene deletion mutant, hemolysin, DNase, and protease production were decreased, whereas protein A expression was increased. The amount of RNAIII, the transcript from the P3 promoter in the agr locus that regulates the expression of various virulence factors, was also reduced in the cvfB mutant. In addition, P2 and P3 promoter activity in the agr locus was decreased in the mutant. Under the genetic background of the agr-null mutation, cvfB gene disruption decreased the production levels of DNase and protease. Moreover, the cvfB and agr double mutant was less virulent than the agr mutant in silkworms. These results suggest that the cvfB gene product contributes to the expression of virulence factors and to pathogenicity via both agr-dependent and agr-independent pathways.
Starting Page	6377
Ending Page	6380
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://iai.asm.org/content/75/4/1964.full.pdf
PubMed reference number	17283102v1
Volume Number	75
Issue Number	4
Journal	Infection and immunity
Language	English
Access Restriction	Open
Subject Keyword	Bombyx Deoxyribonuclease I Endopeptidases Gene Deletion Gene Expression Genome Encoded Entity Hemolysin Mutation Null Value RNAIII, Staphylococcus aureus Transcript Virulence
Content Type	Text
Resource Type	Article