NDLI: Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses

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Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses

Content Provider	Semantic Scholar
Author	Uchugonova, Aisada Breunig, Hans Georg Batista, A. G. Koenig, Karsten
Copyright Year	2015
Abstract	Abstract. We report a virus-free optical approach to human cell reprogramming into induced pluripotent stem cells with low-power nanoporation using ultrashort Bessel-shaped laser pulses. Picojoule near-infrared sub-20 fs laser pulses at a high 85 MHz repetition frequency are employed to generate transient nanopores in the membrane of dermal fibroblasts for the introduction of four transcription factors to induce the reprogramming process. In contrast to conventional approaches which utilize retro- or lentiviruses to deliver genes or transcription factors into the host genome, the laser method is virus-free; hence, the risk of virus-induced cancer generation limiting clinical application is avoided.
File Format	PDF HTM / HTML
DOI	10.1117/1.JBO.20.11.115008
PubMed reference number	26618522
Journal	Medline
Volume Number	20
Alternate Webpage(s)	https://neurophotonics.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.20.11.115008&isResultClick=False
Alternate Webpage(s)	https://journals.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.20.11.115008&isResultClick=False
Alternate Webpage(s)	https://ebooks.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.20.11.115008&isResultClick=False
Alternate Webpage(s)	https://doi.org/10.1117/1.JBO.20.11.115008
Journal	Journal of biomedical optics
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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