NDLI: Factors affecting in vitro adventitious bud induction from excised embryos of Swiss stone pine (Pinus cembra L.)

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Factors affecting in vitro adventitious bud induction from excised embryos of Swiss stone pine (Pinus cembra L.)

Content Provider	Semantic Scholar
Author	Lambardi, Maurizio Capuana, Maurizio Sozzi, Lucia Giannini, Raffaello
Copyright Year	1995
Abstract	Several culture and pre-culture factors affecting ill v i m adventitious bud induction on excised embryos of Swiss stone pine (Pinus cenzbrn L.) were investigatcd. Embryos gave rise to adventitious buds within 4 weeks of culture in a cytokinin-containing medium. Among the tested cytokinins, NLBenzyladenine (BA) proved to bc the most effective, especially when 2.5, or 5 pM were added to the von Arnold and Eriksson's (AE) medium. All the tested pre-induction treatments (4, 8, or 12 week seed chilling. and embryo pre-culture in a hormone-free medium. prior culturing them on the bud induction medium) negligibly affected adventitious budding. Whole embryos were the most suitable explants for bud induction, as poor regeneration was achieved when isolated hypocotyls and epicotyls were cultured. A 21-day exposure of the unchilled explants to BA induced 100% regenerating embryos, and more than 16 buds per explant. Less adventitious shoots were obtained with a 7-day treatment, but shoot growth was moderately accelcrated. Bud development was achieved on a hormone-free AE medium (3 weeks), while shoot elongation was optimized on an activated charcoal-containing (0. 1%) medium (4 weeks). 15 % of the elongated shoots produced adventitious roots, after they were pulsed in liquid l -mM IBA for 5 hours, prior to being transferrred to a hormone-free medium.
Starting Page	49
Ending Page	57
Page Count	9
File Format	PDF HTM / HTML
Volume Number	2
Alternate Webpage(s)	https://kf.tuzvo.sk/sites/default/files/FG02-1_049-058_opravit_na_57.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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