NDLI: Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments.

Content Provider	Semantic Scholar
Author	Yokomaku, D. Yamaguchi, Nobuyasu Nasu, Masao
Copyright Year	2000
Abstract	A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a single freeze-thaw treatment. The number of viable cells was obtained by subtracting the number of remaining cells after the qDVC procedure from the total cell number before the qDVC incubation. This improved procedure should provide useful information about the metabolic potential of natural bacterial communities.
File Format	PDF HTM / HTML
DOI	10.1128/AEM.66.12.5544-5548.2000
Alternate Webpage(s)	http://aem.asm.org/content/66/12/5544.full.pdf
PubMed reference number	11097948
Alternate Webpage(s)	https://doi.org/10.1128/AEM.66.12.5544-5548.2000
Journal	Medline
Volume Number	66
Issue Number	12
Journal	Applied and environmental microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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