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Detección del virus de la leprosis de los cítricos tipo citoplasmático en los Llanos Orientales de Colombia.
| Content Provider | Semantic Scholar |
|---|---|
| Author | León, Guillermo Freitas-Astúa, Juliana Kitajima, Elliot Watanabe Meza, Nora Cristina |
| Copyright Year | 2007 |
| Abstract | Symptoms of citrus leprosis were observed during 2004 - 2005 in the East plains of Colombia, Departments of Meta and Casanare. To identify the causal agent, CORPOICA collected leaves of Valencia sweet orange exhibiting typical lesions of leprosis from several locations in the Departments of Casanare (Yopal, Aguazul) and Meta (Guamal, Villavicencio and Cumaral). Virus identification was done by RT-PCR and transmission electron microscopy (TEM). Ten of the 21 samples examined by TEM resulted positive to the leprosis virus (Citrus Leprosis Virus, CiLV-C). Short bacilliform particles characteristic of CiLV-C were found in the endoplasmic reticulum, and irregularly shaped viroplasms were present in the cytoplasm. Fourteen of the 32 samples processed by RT-PCR were positive to CiLV-C. RT-PCR analyses amplified cDNA fragments of expected size for samples collected in Meta. One of the amplifications was sequenced (GenBank accession No. DQ272491) and found to have 98% nucleotide sequence identity to the Brazilian CiLV-C isolate (GenBank accession No. AY289190.1). Sequence and primers were provided to ICA for CiLV-C diagnosis in Colombia. Mites collected from affected plants were identified as Brevipalpus phoenicis (Geijskes), one of the most important vectors of CiLV-C. |
| Starting Page | 67 |
| Ending Page | 72 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.21930/rcta.vol7_num2_art:72 |
| Alternate Webpage(s) | https://repository.agrosavia.co/bitstream/handle/20.500.12324/18217/42977_48925.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://www.redalyc.org/pdf/4499/449945021008.pdf |
| Alternate Webpage(s) | http://revista.corpoica.org.co/index.php/revista/article/download/72/71 |
| Alternate Webpage(s) | https://doi.org/10.21930/rcta.vol7_num2_art%3A72 |
| Volume Number | 7 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
