Double-degradable responsive self-assembled multivalent arrays--temporary nanoscale recognition between dendrons and DNA.

Content Provider	Semantic Scholar
Author	Barnard, Anna Posocco, Paola Fermeglia, Maurizio Tschiche, Ariane Calderón, Marcelo Pricl, Sabrina Smith, David Addison
Copyright Year	2014
Abstract	This article reports self-assembling dendrons which bind DNA in a multivalent manner. The molecular design directly impacts on self-assembly which subsequently controls the way these multivalent nanostructures bind DNA--this can be simulated by multiscale modelling. Incorporation of an S-S linkage between the multivalent hydrophilic dendron and the hydrophobic units responsible for self-assembly allows these structures to undergo triggered reductive cleavage, with dithiothreitol (DTT) inducing controlled breakdown, enabling the release of bound DNA. As such, the high-affinity self-assembled multivalent binding is temporary. Furthermore, because the multivalent dendrons are constructed from esters, a second slow degradation step causes further breakdown of these structures. This two-step double-degradation mechanism converts a large self-assembling unit with high affinity for DNA into small units with no measurable binding affinity--demonstrating the advantage of self-assembled multivalency (SAMul) in achieving highly responsive nanoscale binding of biological targets.
Starting Page	084501
Ending Page	084501
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://eprints.whiterose.ac.uk/92610/1/Barnard_Smith_OBC2014.pdf
PubMed reference number	24263553v1
Alternate Webpage(s)	https://doi.org/10.1039/c3ob42202j
DOI	10.1039/c3ob42202j
Journal	Organic & biomolecular chemistry
Volume Number	12
Issue Number	3
Language	English
Access Restriction	Open
Subject Keyword	Affinity Catabolism Dendrimers Dithiothreitol Esters Nanostructured Materials
Content Type	Text
Resource Type	Article