NDLI: Label-free and depth resolved optical sectioning of iron-complex deposits in sickle cell disease splenic tissue by multiphoton microscopy

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Label-free and depth resolved optical sectioning of iron-complex deposits in sickle cell disease splenic tissue by multiphoton microscopy

Content Provider	Semantic Scholar
Author	Vigil, Genevieve D. Adami, Alexander J. Ahmed, Tahsin Khan, Aamir A. Chapman, Sarah E. Andemariam, Biree Thrall, Roger S. Howard, Scott S.
Copyright Year	2015
Abstract	Abstract. Multiphoton microscopy (MPM) imaging of intrinsic two-photon excited fluorescence (TPEF) is performed on humanized sickle cell disease (SCD) mouse model splenic tissue. Distinct morphological and spectral features associated with SCD are identified and discussed in terms of diagnostic relevance. Specifically, spectrally unique splenic iron-complex deposits are identified by MPM; this finding is supported by TPEF spectroscopy and object size to standard histopathological methods. Further, iron deposits are found at higher concentrations in diseased tissue than in healthy tissue by all imaging methods employed here including MPM, and therefore, may provide a useful biomarker related to the disease state. These newly characterized biomarkers allow for further investigations of SCD in live animals as a means to gain insight into the mechanisms impacting immune dysregulation and organ malfunction, which are currently not well understood.
File Format	PDF HTM / HTML
DOI	10.1117/1.JBO.20.6.066001
PubMed reference number	26042382
Journal	Medline
Volume Number	20
Alternate Webpage(s)	https://www.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.20.6.066001&isResultClick=False
Alternate Webpage(s)	https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-20/issue-06/066001/Label-free-and-depth-resolved-optical-sectioning-of-iron-complex/10.1117/1.JBO.20.6.066001.pdf
Alternate Webpage(s)	https://neurophotonics.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.20.6.066001&isResultClick=False
Alternate Webpage(s)	https://doi.org/10.1117/1.JBO.20.6.066001
Journal	Journal of biomedical optics
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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