NDLI: Arti ¢ cial chromosome libraries of Streptomyces coelicolor A 3 ( 2 ) and Planobispora rosea 1

Content Provider	Semantic Scholar
Author	Alduina, Rosa Grazia, Simona De Dolce, Luca Salerno, Paola Sosio, Margherita Donadio, Stefano Puglia, Anna Maria
Copyright Year	2003
Abstract	Using an Escherichia coli^Streptomyces shuttle vector derived from a bacterial artificial chromosome (BAC), we developed methodologies for the construction of BAC libraries of filamentous actinomycetes. Libraries of Streptomyces coelicolor, the model actinomycete, and Planobispora rosea, a genetically intractable strain, were constructed. Both libraries have an average insert size of 60 kb, with maximal insert larger than 150 kb. The S. coelicolor library was evaluated by selected hybridisations to DraI fragments and by end sequencing of a few clones. Hybridisation of the P. rosea library to selected probes indicates a good representation of the P. rosea genome and that the library can be used to facilitate the genomic analysis of this actinomycete. : 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://iris.unipa.it/retrieve/handle/10447/286039/560985/2003_FEMS.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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