Identifizierung und Charakterisierung eines neuen Clusters von T-Zell-Rezeptor Delta-Rekombinationselementen

Content Provider	Semantic Scholar
Author	Wanzeck, Jens
Copyright Year	2005
Abstract	1 Abstrakt Es konnte ein neuer Cluster von insgesamt sieben T-Zell-Rezeptor δ-Rekombinationselementen genutzt werden. Abstract 2 Abstract A new cluster of 7 T-cell receptor δ recombining elements (δRec2.1-2.7) located within the T-cell receptor δ (TCRD) gene, 2.6-5.2 kb downstream from the variable region (Vδ2) gene segment could be identified. The δRec2 elements are isolated recombination signal sequences to rearrange with the Dδ3, Jδ1 segments of the TCRD gene as well as with the pseudo joining segment of the TCRA gene. Rearrangements involving the δRec2 elements were found in all peripheral blood (PB) samples from 10 healthy individuals, although their frequency was about 100-fold lower than classical δRec rearrangements. Compared with thymocytes, the total frequency of δRec2 rearrangements was lower in PB T-cells, suggesting that they are deleted during T-cell development. The decrease in frequency of the δRec2-Dδ3 rearrangements was most striking: 11 times lower in PB T lymphocytes than in thymocytes. Since the δRec2-Jδ1 rearrangements contained the Dδ3 segment in the junctional region, it could be assumed that they are derived from the δRec2-Dδ3 rearrangements. In contrast, the majority of δRec2-ψJα rearrangements did not contain the Dδ3 segment, indicating that they are single step rearrangements. The δRec2-Jδ1 and δRec2-ψJα rearrangements seem to be T-lineage specific, but the δRec2-Dδ3 rearrangements were also found at very low frequencies in B-lymphocytes and NK-cells. The results suggest that δRec2 rearrangements are transient steps in the recombinatorial process of the TCRAD locus and are probably deleted by subsequent Vα-Jα rearrangements. Similarly to the classical δRec rearrangements, also the δRec2 rearrangements most likely play a role in T-cell differentiation towards the TCRαβ lineage. These recombination elements could also complete the rearrangements used so far as marker in diagnosis of minimal residual disease of malignant lymphatic diseases.
File Format	PDF HTM / HTML
DOI	10.18452/15319
Alternate Webpage(s)	https://edoc.hu-berlin.de/bitstream/handle/18452/15971/Wanzeck.pdf?isAllowed=y&sequence=1
Alternate Webpage(s)	http://edoc.hu-berlin.de/dissertationen/wanzeck-jens-2005-07-18/PDF/Wanzeck.pdf
Alternate Webpage(s)	https://doi.org/10.18452/15319
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article