The abundance of sterile transcripts in Giardia lamblia.

Content Provider	Semantic Scholar
Author	Elmendorf, Heidi G. Singer, Steven M. Nash, Theodore Elliott
Copyright Year	2001
Abstract	The protozoan parasite Giardia lamblia synthesizes a diverse and surprisingly abundant array of sterile transcripts unable to code for proteins. Random sampling of cDNAs from two evolutionarily divergent Giardia strains indicates that approximately 20% of cDNAs in the libraries represent polyadenylated sterile transcripts. RNase protection analysis and northern blot hybridization of three sterile transcript loci demonstrated that both the sterile transcript and a complementary mRNA were made in each case, further categorizing these sterile transcripts as antisense transcripts. Investigation of the genomic loci for these same three sterile antisense transcripts showed typical transcription units for the sense transcripts, but still failed to reveal a usable open reading frame for the sterile antisense transcripts. 5'-RACE mapped the transcription start site for one of the sterile antisense transcripts to an AT-rich region, as is typical for GIARDIA: It is unclear whether these sterile transcripts represent errors in transcription or whether they have regulatory functions within the cell, although preliminary investigations failed to reveal evidence for a role in developmental gene regulation. In either case, the presence of such a large pool of sterile antisense transcripts is dramatic evidence of the unusual molecular machinery of the early diverging protist G.lamblia.
Starting Page	446
Ending Page	448
Page Count	3
File Format	PDF HTM / HTML
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PubMed reference number	11713317v1
Volume Number	29
Issue Number	22
Journal	Nucleic acids research
Language	English
Access Restriction	Open
Subject Keyword	Giardia lamblia Infertility Northern Blotting Nucleic Acid Hybridization Open Reading Frames Poly A Protozoa Reading Frames (Nucleotide Sequence) Reticulitermes speratus gut symbiotic protist Transcript Transcription Initiation Site antisense therapy mapped protists
Content Type	Text
Resource Type	Article