Shewanella Oneidensis MR-1 Msh Pilin Proteins are Involved in Extracellular Electron Transfer in Microbial Fuel Cells

Content Provider	Semantic Scholar
Author	Fitzgerald, Lisa A. Petersen, Emily R. Ray, Richard I. Little, Brenda J. Cooper, Candace J. Howard, Erinn C. Ringeisen, Bradley R. Biffinger, Justin C.
Copyright Year	2012
Abstract	Shewanella is a microbial genus that can oxidize lactate for the reduction of insoluble electron acceptors. This reduction is possible by either direct (cell–surface interaction, nanowires) or indirect (soluble redox mediators) mechanisms. However, the actual molecular identification of a nanowire has not been determined. Through mutational studies, Shewanella oneidensis MR-1 was analyzed for its ability to transfer electrons to an electrode after deletion of the structural pilin genes (ΔmshA-D) or the entire biosynthetic expression system (ΔmshH-Q) of one of its pilin complexes (Msh type IV pilus gene locus). The complete removal of the Msh complex (ΔmshH-Q) significantly decreased the current generated from a fuel cell compared to MR-1. However, the mutant with only extracellular Msh structural proteins removed (ΔmshA-D) was able to generate 80% of the current compared to MR-1. Thus, the intracellular and membrane bound Msh biogenesis complex is a pathway for extracellular electron transfer in S. oneidensis MR-1.
Starting Page	170
Ending Page	174
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1016/j.procbio.2011.10.029
Volume Number	47
Alternate Webpage(s)	http://www.dtic.mil/dtic/tr/fulltext/u2/a553026.pdf
Alternate Webpage(s)	https://frontis-energy.com/wp-content/uploads/2018/05/electron_transfer/Shewanella-%20MR-1%20pilin%20proteins%20are%20involved%20in%20extracellular%20transfer.%20Fitzgerald%20etal.%20(Biffenger).%20ProcBiochem.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.procbio.2011.10.029
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article