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Ubiquitin promoter–terminator cassette promotes genetically stable expression of the taste-modifying protein miraculin in transgenic lettuce
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hirai, Tadayoshi Shohael, Abdullah Mohammad Kim, You-Wang Yano, Megumu Ezura, Hiroshi |
| Copyright Year | 2011 |
| Abstract | Lettuce is a commercially important leafy vegetable that is cultivated worldwide, and it is also a target crop for plant factories. In this study, lettuce was selected as an alternative platform for recombinant miraculin production because of its fast growth, agronomic value, and wide availability. The taste-modifying protein miraculin is a glycoprotein extracted from the red berries of the West African native shrub Richadella dulcifica. Because of its limited natural availability, many attempts have been made to produce this protein in suitable alternative hosts. We produced transgenic lettuce with miraculin gene driven either by the ubiquitin promoter/terminator cassette from lettuce or a 35S promoter/nos terminator cassette. Miraculin gene expression and miraculin accumulation in both cassettes were compared by quantitative real-time PCR analysis, Western blotting, and enzyme-linked immunosorbent assay. The expression level of the miraculin gene and protein in transgenic lettuce was higher and more genetically stable in the ubiquitin promoter/terminator cassette than in the 35S promoter/nos terminator cassette. These results demonstrated that the ubiquitin promoter/terminator cassette is an efficient platform for the genetically stable expression of the miraculin protein in lettuce and hence this platform is of benefit for recombinant miraculin production on a commercial scale. |
| Starting Page | 2255 |
| Ending Page | 2265 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s00299-011-1131-x |
| PubMed reference number | 21830129 |
| Journal | Medline |
| Volume Number | 30 |
| Alternate Webpage(s) | https://tsukuba.repo.nii.ac.jp/index.php?action=pages_view_main&active_action=repository_action_common_download&attribute_id=17&block_id=83&file_no=1&item_id=25848&item_no=1&page_id=13 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00299-011-1131-x |
| Journal | Plant Cell Reports |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
