Antibodies to a nucleolar protein are localized in the nucleolus after red blood cell-mediated microinjection

Content Provider	Semantic Scholar
Author	Bennett, Forrest C. Busch, Harris Lischwe, Michael A. Yeoman, Lynn C.
Copyright Year	1983
Abstract	To determine whether red blood cell-mediated microinjection of antibodies can be used to study nuclear protein localization and function, we microinjected antibodies that have been shown to react specifically with nucleolar acidic phosphoprotein C23 into Walker 256 cells. The intracellular distribution of microinjected anti-C23 antibodies and preimmune immunoglobulins were determined by immunofluorescence. At 3 h after microinjection, affinity-purified anti-C23 antibodies were localized in the cytoplasm and nucleolus. At 17 h after microinjection, the affinity-purified antibody was localized to those nucleolar structures previously shown to contain protein C23. Furthermore, the antibody remained localized in the nucleolus for at least 36 h after microinjection. In contrast to the results obtained with specific antibodies, preimmune immunoglobulins remained in the cytoplasm 36 h after microinjection. These results indicate that red blood cell-mediated microinjection of antibodies can be used to study nucleolar and nuclear antigens.
Starting Page	1566
Ending Page	1572
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	6355122
Volume Number	97
Journal	The Journal of cell biology
Language	English
Access Restriction	Open
Subject Keyword	ANP32B protein, human Acids Affinity Antigens, Nuclear Blood Cells Cell Nucleolus Erythrocytes Fluorescent Antibody Technique Immunoglobulins Microinjections NCL gene Nuclear Proteins Phosphoproteins Tritium intracellular protein transport
Content Type	Text
Resource Type	Article