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Peptide nucleic acid-ionic self-complementary peptide conjugates: highly efficient DNA condensers with specific condensing mechanism
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cao, Meiwen Zhao, Wenjing Zhou, Peng Xie, Zilong Sun, Yawei |
| Copyright Year | 2017 |
| Abstract | A new type of conjugated molecule including T′3–(AKAE)2, T′3–(IKIE)2, and A′3–(AKAE)2 was designed by linking short peptide nucleic acid (PNA) segments with short ionic self-complementary peptide (ISCP) sequences. These short conjugates showed high hybridization affinity and specificity for λ-DNA. They can induce efficient DNA condensation at low micromole concentrations via a specific mechanism that involves the base pair recognition between DNA and the PNA segment and the self-aggregation of the bound PNA–ISCP molecules. Atomic force microscopy (AFM) and dynamic light scattering (DLS) measurements indicated that λ-DNA took an elongated conformation while it compacted into globules when interacting with the PNA–ISCP conjugates. The ethidium bromide displacement assay indicated that the PNA–ISCP conjugates induced DNA condensation in a way different from conventional cationic condensers such as polyethyleneimine (PEI) and hexadecyltrimethylammonium bromide (CTAB). The interaction between T′3–(AKAE)2 and a single chain oligonucleotide, d(A)36, was further studied and the results revealed that the PNA–ISCP conjugates bound with DNA mainly via base pairing recognition. The volume ratio of λ-DNA and the λ-DNA/PNA–ISCP globules was calculated based on AFM measurements, which was near 1 : 1, suggesting that the condensation was an intramolecular folding process for λ-DNA, which was prompted by the self-aggregation of the bound PNA–ISCP molecules. |
| Starting Page | 3796 |
| Ending Page | 3803 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C6RA26329A |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/c6/ra/c6ra26329a/c6ra26329a1.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/C6RA26329A |
| Volume Number | 7 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
