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Monoclonal antibodies to a particulate superoxide-forming system stimulate a respiratory burst in intact guinea pig neutrophils.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Berton, Giorgio Rosen, H. K. Ezekowitz, R. . Alan B. Bellavite, Paolo Serra, Maria Cristina Rossi, Ferdinand Gordon, Saimon |
| Copyright Year | 1986 |
| Abstract | Monoclonal rat antibodies were produced against a subcellular preparation of phorbol 12-myristate 13-acetate (PMA)-stimulated guinea pig neutrophils that retains NADPH-oxidase activity. Two antibodies, 1A10.4 and IG4, were isolated that bind to a surface antigen restricted to guinea pig neutrophils from bone marrow and peritoneal exudate and to macrophages and that trigger a respiratory burst in neutrophils in the presence of cytochalasin B. Intact antibody 1A10.4, subclass IgG2c, can trigger superoxide anion release directly; F(ab')2 fragments of 1A10.4 and intact IG4 require further cross-linking by F(ab')2 fragments of anti-rat immunoglobulin antibody. Both antibodies recognize the same antigen, a proteolipid of apparent molecular mass 10 kDa. Immunoprecipitation of solubilized oxidase activity with 1A10.4 brings down this activity as part of a macromolecular complex. Surface expression of the antigen is increased on treatment of cells with both PMA and cytochalasin B. 1A10.4 also triggers release of the granule enzyme beta-glucuronidase. Triggering of a respiratory burst by the antibodies appears distinct from the PMA and fMet-Leu-Phe signalling systems. These studies indicate that the antigen defined by antibodies 1A10.4 and IG4 becomes associated with the superoxide anion-generating system of neutrophils but may play a more general role in signal transduction in phagocytic cells. |
| Starting Page | 1 |
| Ending Page | 2 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.paolobellavite.it/files/055_1986_monoclonalnadphoxpnas.pdf |
| PubMed reference number | 3012541v1 |
| Volume Number | 83 |
| Issue Number | 11 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anions Bone Marrow Cavia porcellus Cytochalasins Exudate Hepatitis B Surface Antigens Leucine Molecular Mass Particulate (substance) Phenylalanine Phorbols Precipitating Factors Proteolipids Respiratory Burst Signal Transduction Superoxides biological signaling fMet-Leu-Phe receptor subclass |
| Content Type | Text |
| Resource Type | Article |
