NDLI: Leinamycin biosynthesis revealing unprecedented architectural complexity for a hybrid polyketide synthase and nonribosomal peptide synthetase.

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Leinamycin biosynthesis revealing unprecedented architectural complexity for a hybrid polyketide synthase and nonribosomal peptide synthetase.

Content Provider	Semantic Scholar
Author	Tang, Gong-Li Cheng, Y. S. Shen, Ben
Copyright Year	2004
Abstract	A 135,638 bp DNA region that encompasses the leinamycin (LNM) biosynthetic gene cluster was sequenced from Streptomyces atroolivaceus S-140. The boundaries of the lnm cluster were defined by systematic inactivation of open reading frames within the sequenced region. The lnm cluster spans 61.3 kb of DNA and consists of 27 genes encoding nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS), hybrid NRPS-PKS, resistance, regulatory, and tailoring enzymes, as well as proteins of unknown function. A model for LNM biosynthesis is proposed, central to which is the LNM hybrid NRPS-PKS megasynthetase consisting of discrete (LnmQ and LnmP) and modular (LnmI) NRPS, acyltransferase-less PKS (LnmG, LnmI, and LnmJ), and PKS modules with unusual domain organization. These studies unveil an unprecedented architectural complexity for the LNM hybrid NRPS-PKS megasynthetase and set the stage to investigate the molecular basis for LNM biosynthesis.
Starting Page	33
Ending Page	45
Page Count	13
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www4.uwm.edu/letsci/biologicalsciences/facultystaff/cheng/upload/5-Cheng_C-B2004.pdf
Alternate Webpage(s)	https://cms.uwm.edu/letsci/biologicalsciences/facultystaff/cheng/upload/5-Cheng_C-B2004.pdf
Alternate Webpage(s)	http://www4dev.uwm.edu/letsci/biologicalsciences/facultystaff/cheng/upload/5-Cheng_C-B2004.pdf
PubMed reference number	15112993v1
Volume Number	11
Issue Number	1
Journal	Chemistry & biology
Language	English
Access Restriction	Open
Subject Keyword	Acyltransferase Anabolism Frame (physical object) Ligase Open Reading Frames Reading Frames (Nucleotide Sequence) leinamycin nonribosomal peptide synthetase polyketide synthase
Content Type	Text
Resource Type	Article

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