Actin filament assembly by myristoylated alanine-rich C kinase substrate-phosphatidylinositol-4,5-diphosphate signaling is critical for dendrite branching.

Content Provider	Semantic Scholar
Author	Li, Haimin Chen, Gang Zhou, Bing Duan, Shumin
Copyright Year	2008
Abstract	Dendrites undergo extensive growth and branching at early stages, but relatively little is known about the molecular mechanisms underlying these processes. Here, we show that increasing the level of myristoylated, alanine-rich C kinase substrate (MARCKS), a prominent substrate of protein kinase C and a phosphatidylinositol-4,5-diphosphate [PI(4,5)P2] sequestration protein highly expressed in the brain, enhanced branching and growth of dendrites both in vitro and in vivo. Conversely, knockdown of endogenous MARCKS by RNA interference reduced dendritic arborization. Results from expression of different mutants indicated that membrane binding is essential for MARCKS-induced dendritic morphogenesis. Furthermore, MARCKS increased the number and length of filamentous actin-based filopodia along neurites, as well as the motility of filopodia, in a PI(4,5)P2-dependent manner. Time-lapse imaging showed that MARCKS increased frequency of filopodia initiation but did not affect filopodia longevity, suggesting that MARCKS may increase dendritic branching through its action on filopodia initiation. These findings demonstrate a critical role for MARCKS-PI(4,5)P2 signaling in regulating dendrite development.
File Format	PDF HTM / HTML
DOI	10.1091/mbc.E08-03-0294
PubMed reference number	18799624
Journal	Medline
Volume Number	19
Issue Number	11
Alternate Webpage(s)	http://www.molbiolcell.org/content/19/11/4804.full.pdf
Alternate Webpage(s)	http://ir.sibs.ac.cn/bitstream/331001/1695/2/Li-2008-Actin%20Filament%20Assem.pdf
Alternate Webpage(s)	https://doi.org/10.1091/mbc.E08-03-0294
Journal	Molecular biology of the cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article