Loading...
Please wait, while we are loading the content...
Novel interaction between the M4 muscarinic acetylcholine receptor and elongation factor 1A2.
| Content Provider | Semantic Scholar |
|---|---|
| Author | McClatchy, Daniel B. Knudsen, Charlotte Rohde Clark, Brian F. C. Kahn, Richard A. Hall, Randy A. Levey, Allan I. |
| Copyright Year | 2002 |
| Abstract | The activation of the muscarinic acetylcholine receptor (mAChR) family, consisting of five subtypes (M1-M5), produces a variety of physiological effects throughout the central nervous system. However, the role of each individual subtype remains poorly understood. To further elucidate signal transduction pathways for specific subtypes, we used the most divergent portion of the subtypes, the intracellular third (i3) loop, as bait to identify interacting proteins. Using a brain pull-down assay, we identify elongation factor 1A2 (eEF1A2) as a specific binding partner to the i3 loop of M4, and not to M1 or M2. In addition, we demonstrate a direct interaction between these proteins. In the rat striatum, the M4 mAChR colocalizes with eEF1A2 in the soma and neuropil. In PC12 cells, endogenous eEF1A2 co-immunoprecipitates with the endogenous M4 mAChR, but not with the endogenous M1 mAChR. In our in vitro model, M4 dramatically accelerates nucleotide exchange of eEF1A2, a GTP-binding protein. This indicates the M4 mAChR is a guanine exchange factor for eEF1A2. eEF1A2 is an essential GTP-binding protein for protein synthesis. Thus, our data suggest a novel role for M4 in the regulation of protein synthesis through its interaction with eEF1A2. |
| Starting Page | 1 |
| Ending Page | 2 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://pharmacology.emory.edu/hall/publications/McClatchy_et_al_2002.pdf |
| Alternate Webpage(s) | http://www.pharm.emory.edu/rhall/McClatchy_et_al_2002.pdf |
| Alternate Webpage(s) | http://www.pharm.emory.edu/hall/publications/McClatchy_et_al_2002.pdf |
| PubMed reference number | 12048193v1 |
| Volume Number | 277 |
| Issue Number | 32 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CNS disorder Cell body of neuron Cholinergic Receptors Codependency (Psychology) EEF1A2 protein, human Guanine Nucleotide Exchange Factors Guanosine Triphosphate In Vitro [Publication Type] M4 Additive/Preservative Muscarinic Acetylcholine Receptor Neostriatum Nucleotides Protein Biosynthesis Signal Transduction Pathways Subtype (attribute) |
| Content Type | Text |
| Resource Type | Article |
