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Anti-inflammatory and Anti-oxidant Studies of Osung-tang Extracts in LPS-Induced RAW 264.7 Cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kim, Sojeong Kim, Tae-Jun Min, Kim Yong |
| Copyright Year | 2020 |
| Abstract | Objectives : The purpose of this study was to investigate the anti-inflammatory and anti-oxidative effects of Osung–tang(OST) extract. Methods : MTT assay was performed to confirm the survival rate of RAW 264.7 cells treated with OST extract(50-400μg/ml) and the production of NO from LPS-induced RAW 264.7 cells was confirmed. The mRNA expression of iNOS, COX-2, and NF-κB were measured by real-time PCR. The protein expression of iNOS, COX-2 and p-IκB were measured by western blot and the anti-oxidant activity of OST extract(50-400μg/ml) was investigated by measuring DPPH scavenging activity. Results : OST extract showed a cell survival rate of 90% or more at 50-400μg/ml. The NO production was inhibited in a dose-dependent manner in RAW 264.7 cells treated with LPS on OST treated group. mRNA expression levels of iNOS, COX-2 and NF-κB decreased in a concentration-dependent manner after treatment with OST(50-400μg/ml). Protein expression levels of iNOS, COX-2 and p-IκB decreased in a concentration-dependent manner after treatment with OST(50-400μg/ml). It was found that OST has a high free-radical scavenging ability. c 2020 the Society of Korean Medicine Ophthalmology & Otolaryngology & Dermatology This is an Open Access journal distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/license/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 한방안이비인후피부과학회지 제33권 제1호(2020년 2월) |
| Starting Page | 1 |
| Ending Page | 11 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.6114/jkood.2020.33.1.001 |
| Volume Number | 33 |
| Alternate Webpage(s) | https://www.jkmood.org/download/download_pdf?pid=jkmood-33-1-1 |
| Alternate Webpage(s) | https://doi.org/10.6114/jkood.2020.33.1.001 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
