Gene expression in human embryonic stem cell lines: unique molecular signature.

Content Provider	Semantic Scholar
Author	Bhattacharya, Bhaskar Miura, Takumi Brandenberger, Ralph Mejido, Josef Luo, Yongquan Yang, Amy X. Joshi, Bharat H. Ginis, Irene Thies, R. Scott Amit, Michal Lyons, Ian Condie, Brian Gene Itskovitz-Eldor, Joseph Rao, Mahendra S. Puri, Raj K.
Copyright Year	2004
Abstract	Human embryonic stem (huES) cells have the ability to differentiate into a variety of cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. However, little is known about the mechanism of differentiation of huES cells and factors regulating cell development. We have used high-quality microarrays containing 16 659 seventy-base pair oligonucleotides to examine gene expression in 6 of the 11 available huES cell lines. Expression was compared against pooled RNA from multiple tissues (universal RNA) and genes enriched in huES cells were identified. All 6 cell lines expressed multiple markers of the undifferentiated state and shared significant homology in gene expression (overall similarity coefficient > 0.85).A common subset of 92 genes was identified that included Nanog, GTCM-1, connexin 43 (GJA1), oct-4, and TDGF1 (cripto). Gene expression was confirmed by a variety of techniques including comparison with databases, reverse transcriptase-polymerase chain reaction, focused cDNA microarrays, and immunocytochemistry. Comparison with published "stemness" genes revealed a limited overlap, suggesting little similarity with other stem cell populations. Several novel ES cell-specific expressed sequence tags were identified and mapped to the human genome. These results represent the first detailed characterization of undifferentiated huES cells and provide a unique set of markers to profile and better understand the biology of huES cells.
Starting Page	297
Ending Page	308
Page Count	12
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.bloodjournal.org/content/bloodjournal/103/8/2956.full.pdf?sso-checked=true
PubMed reference number	15070671v1
Volume Number	103
Issue Number	8
Journal	Blood
Language	English
Access Restriction	Open
Subject Keyword	Base Pairing Body tissue Cell development Connexins Cultured Cell Line DNA, Complementary Embryonic Stem Cells GJA1 gene Gene Expression Homologous Gene Immunocytochemistry Intersex Molecular Profiling Oligonucleotides Pierre Robin Syndrome RNA RNA-Directed DNA Polymerase Reverse Transcriptase Polymerase Chain Reaction Scientific Publication Subgroup TDGF1 gene
Content Type	Text
Resource Type	Article