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Cytotoxic and Apoptotic Effect of Annona Squamosa Seed Extract on Human and Murine Tumor Cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sobhana, Biba Veerabhadran Prathapan, Remani |
| Copyright Year | 2013 |
| Abstract | Background/Aim: The aim of this study was to evaluate the cytotoxic and apoptotic effects of chloroform extract (ASCH) from the seeds of Annona squamosa. Materials and Methods: MTT assay was employed to determine the cytotoxicity of the extract on A-549 and EAC cells and IC50 values were calculated. The apoptotic effects induced by the ASCH was evaluated by Acridine Orange - Ethidium Bromide dual staining and Hoechst staining by Fluorescence microscopy. Results: The ASCH extract 400 µg/ml exhibited maximum cytotoxic effects of 54±1.2 and 56± 0.9 at 48 hours on human Lung adenocarcinoma cells (A-549) and murine tumor (EAC) cells respectively. IC50 values were 319 µg/ml and 312 µg/ml for A-549 cells and EAC cells respectively. Acridine Orange Ethidium Bromide and Hoechst staining exhibited apoptotic features such as chromatin condensation and nuclear fragmentation after treatment with the extract. Conclusions: These results suggest that the chloroform extract from Annona squamosa seeds have cytotoxic and apoptotic effect. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ijpbs.net/cms/php/upload/2876_pdf.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
