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Biotransformation of p-xylene into terephthalic acid by engineered Escherichia coli
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lee, Sang Yup |
| Copyright Year | 2017 |
| Abstract | Terephthalic acid (TPA) is an important industrial chemical currently produced by energy intensive and potentially hazardous p-xylene (pX) oxidation process. Here we report the development of metabolically engineered Escherichia coli system for biological transformation of pX into TPA. The engineered E. coli strain harbours a synthetic TPA pathway optimized through manipulation of expression levels of upstream and downstream modules. The upstream pathway converts pX to p-toluic acid (pTA) and the downstream pathway transforms pTA to TPA. In a two-phase partitioning fermentation, the engineered strain converts 8.8 g pX into 13.3 g TPA, which corresponds to a conversion yield of 96.7 mol%. These results suggest that the E. coli system presented here might be a promising alternative for the large-scale biotechnological production of TPA and lays the foundations for the future development of sustainable approaches for TPA production. |
| File Format | PDF HTM / HTML |
| DOI | 10.1038/ncomms15689 |
| PubMed reference number | 28561070 |
| Journal | Medline |
| Volume Number | 8 |
| Alternate Webpage(s) | http://koasas.kaist.ac.kr/bitstream/10203/224058/1/000402395800001.pdf |
| Alternate Webpage(s) | https://doi.org/10.1038/ncomms15689 |
| Journal | Nature communications |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
