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Congenital toxoplasmosis: evaluation of serological methods for the detection of anti-Toxoplasma gondii IgM and IgA antibodies.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rodrigues, Isolina Maria Xavier Castro, Ana María Gomes, Maria Flávia Belfort Amaral, Waldemar Naves Do Avelino, Mariza Martins |
| Copyright Year | 2009 |
| Abstract | A study was carried out to evaluate the presence of serological markers for the immunodiagnosis of the vertical transmission of toxoplasmosis. We tested the sensitivity, specificity and predictive values (positive and negative) of different serological methods for the early diagnosis of congenital toxoplasmosis. In a prospective longitudinal study, 50 infants with suspected congenital toxoplasmosis were followed up in the ambulatory care centre of Congenital Infections at University Hospital in Goiânia, Goiás, Brazil, from 1 January 2004-30 September 2005. Microparticle Enzyme Immunoassay (MEIA), Enzyme-Linked Fluorescent Assay (ELFA) and Immune-Fluorescent Antibody Technique (IFAT) were used to detect specific IgM anti-Toxoplasma gondii antibodies and a capture ELISA was used to detect specific IgA antibodies. The results showed that 28/50 infants were infected. During the neonatal period, IgM was detected in 39.3% (11/28) of those infected infants and IgA was detected in 21.4% (6/28). The sensitivity, specificity and predictive values (positive and negative) of each assay were, respectively: MEIA and ELFA: 60.9%, 100%, 100%, 55.0%; IFAT: 59.6%, 91.7%, 93.3%, 53.7%; IgA capture ELISA: 57.1%, 100%, 100%, 51.2%. The presence of specific IgM and IgA antibodies during the neonatal period was not frequent, although it was correlated with the most severe cases of congenital transmission. The results indicate that the absence of congenital disease markers (IgM and IgA) in newborns, even after confirming the absence with several techniques, does not constitute an exclusion criterion for toxoplasmosis. |
| Starting Page | 278 |
| Ending Page | 281 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.scielo.br/pdf/mioc/v104n3/06.pdf |
| PubMed reference number | 19547868v1 |
| Volume Number | 104 |
| Issue Number | 3 |
| Journal | Memorias do Instituto Oswaldo Cruz |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Accident and Emergency department Cell-Derived Microparticles Congenital Disorders Congenital Heart Defects Enzyme-Linked Immunosorbent Assay Exclusion Fluorescent Antibody Technique Hip Dislocation, Congenital Immunoglobulin A Immunoglobulin M Infant, Newborn Microparticle Enzyme Immunoassay Toxoplasma gondii Toxoplasmosis Toxoplasmosis, Congenital Vertical Disease Transmission ambulatory care services |
| Content Type | Text |
| Resource Type | Article |
