NDLI: Highly luminescent, biocompatible ytterbium(iii) complexes as near-infrared fluorophores for living cell imaging† †Electronic supplementary information (ESI) available: Detailed photophysical and cell experiment data; 1H and 19F NMR spectra, UV-visible absorption spectra; IR spectra and MS spectra.

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Highly luminescent, biocompatible ytterbium(iii) complexes as near-infrared fluorophores for living cell imaging† †Electronic supplementary information (ESI) available: Detailed photophysical and cell experiment data; 1H and 19F NMR spectra, UV-visible absorption spectra; IR spectra and MS spectra.

Content Provider	Semantic Scholar
Author	Ning, Yingying Tang, Juan Jing, Jing Sun, Yuansheng Zhang, Jun-Long
Copyright Year	2018
Abstract	Herein, we report the design and synthesis of biocompatible Yb3+ complexes for near-infrared (NIR) living cell imaging. Upon excitation at either the visible (Soret band) or red region (Q band), these β-fluorinated Yb3+ complexes display high NIR luminescence (quantum yields up to 23% and 13% in dimethyl sulfoxide and water, respectively) and have higher stabilities and prolonged decay lifetimes (up to 249 μs) compared to the β-non-fluorinated counterparts. This renders the β-fluorinated Yb3+ complexes as a new class of biological optical probes in both steady-state imaging and time-resolved fluorescence lifetime imaging (FLIM). NIR confocal fluorescence images showed strong and specific intracellular Yb3+ luminescence signals when the biocompatible Yb3+ complexes were uptaken into the living cells. Importantly, FLIM measurements showed an intracellular lifetime distribution between 100 and 200 μs, allowing an effective discrimination from cell autofluorescence, and afforded high signal-to-noise ratios as firstly demonstrated in the NIR region. These results demonstrated the prospects of NIR lanthanide complexes as biological probes for NIR steady-state fluorescence and time-resolved fluorescence lifetime imaging.
Starting Page	3742
Ending Page	3753
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1039/c8sc00259b
PubMed reference number	29780506
Journal	Medline
Volume Number	9
Alternate Webpage(s)	http://pubs.rsc.org/en/content/articlepdf/2018/sc/c8sc00259b
Alternate Webpage(s)	http://www.rsc.org/suppdata/c8/sc/c8sc00259b/c8sc00259b1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/c8sc00259b
Journal	Chemical science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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