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Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kuo, Soong Yu Chien, Lee Feng Hsiao, Yi Yuong Ru, Chuan Van Yan, Kun Huang Liu, Pei Feng Mao, Simon Jen-Tan Pan, Rong Long |
| Copyright Year | 2005 |
| Abstract | Endoplasmic reticulum (ER)-enriched vesicles from etiolated hypocotyls of mung bean seedlings (Vigna radiata) were successfully isolated using Ficoll gradient and two-phase (polyethylene glycol-dextran) partition. The ER-enriched vesicles contained inorganic pyrophosphate (PPi) hydrolysis and its associated proton translocating activities. Antiserum prepared against vacuolar H+-pyrophosphatase (V-PPase, EC 3.6.1.1) did not inhibit this novel pyrophosphatase-dependent proton translocation, excluding the possible contamination of tonoplast vesicles in the ER-enriched membrane preparation. The optimal ratios of Mg2+/PPi (inorganic pyrophosphate) for enzymatic activity and PPi-dependent proton translocation of ER-enriched vesicles were higher than those of vacuolar membranes. The PPi-dependent proton translocation of ER-enriched vesicles absolutely required the presence of monovalent cations with preference for K+, but could be inhibited by a common PPase inhibitor, F-. Furthermore, ER H+-pyrophosphatase exhibited some similarities and differences to vacuolar H+-PPases in cofactor/substrate ratios, pH profile, and concentration dependence of F-, imidodiphosphate (a PPi analogue), and various chemical modifiers. These results suggest that ER-enriched vesicles contain a novel type of proton-translocating PPase distinct from that of tonoplast from higher plants. |
| Starting Page | 843 |
| Ending Page | 849 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://ir.nctu.edu.tw/bitstream/11536/23799/1/000227721100001.pdf |
| PubMed reference number | 15779823v1 |
| Volume Number | 162 |
| Issue Number | 2 |
| Journal | Journal of plant physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Analog Cations Cations, Monovalent Contain (action) Dextrans Endoplasmic Reticulum Ficoll Gradient Hypocotyl PPA1 gene Polyethylene Glycols Potassium Proton-Phosphate Symporters Proton-Translocating ATPases Protons Seedlings Tissue membrane Vesicle (morphologic abnormality) Vigna radiata imidodiphosphate plant-type vacuole membrane proton transport proton-translocating pyrophosphatase pump (device) |
| Content Type | Text |
| Resource Type | Article |
