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Highly sensitive gold nanoparticle based electrochemical biosensor for detection of Antigen antibody interactions
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rawat, Anita Singh, Karan Pratap Vasmatkar, Pashupat Baral, Pratibha |
| Copyright Year | 2012 |
| Abstract | The electrochemical biosensor was designed for label-free detection of bovine serum albumin (BSA). In the developed electrochemical sensor gold coated polycarbonate membrane of different 30, 50 and 100 nm pore sizes. were used for detection. The gold coated polycarbonate membranes were thiolated by 16-Mercaptohexadecanoic acid (MHDA) and then activated by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) for the binding of the antibody (BSA). After activation of the membranes, the BSA antibody was immobilized and the membranes were subjected to the detection of BSA antigen with the help of a homemade electrochemical setup. The specifi city of the antibody was cross-checked with a non-corresponding Prostate Specifi c Antigen (PSA). The specifi city and sensitivity of the designed biosensor along with the signal amplifi cation due to binding of antigen-gold nanoparticle conjugate was also determined. The impedance corresponding to each step of membrane modifi cation was observed and it was found that there was a sharp increase in the measured impedance from modifi cation to detection. Also, there was multiple fold increase in signal due to tagging of GNP. The impedance corresponding to different pore sized membranes was used to fi nd a suitable pored membrane for the sensitive detection and it was observed that 30 nm pore size membrane showed comparatively better result than 50 and 100 nm pore size membrane. |
| File Format | PDF HTM / HTML |
| DOI | 10.21786/bbrc/10.1/13 |
| Alternate Webpage(s) | http://bbrc.in/bbrc/2017jan-marchPDF/BBRC11_013.pdf |
| Alternate Webpage(s) | https://doi.org/10.21786/bbrc%2F10.1%2F13 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
