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HPLC Determination of Methylxanthines and Polyphenols Levels In Cocoa and Chocolate Products
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ramli, Nazaruddin Yatim, Ayub Mohd Hok, Heng Cheng |
| Copyright Year | 2001 |
| Abstract | Commercial cocoa and chocolate products such as cocoa powder, cocoa beans, cocoa liquor and chocolate were analyzed for total polyphenols, (-)-epicatechin, (+)-catechin, theobromine and caffeine contents by High Performance Liquid Chromatography (HPLC). The methylxanthines were identified and quantified with the use of -Bondapak column and mobile phase of methanol:water:acetic acid (20:79:1). C.V. values were relatively low ( 90%). Total polyphenols ranged from 45 52 mg/g in cocoa liquor, 34 60 mg/g cocoa beans, 20 62 mg/g in cocoa powder. For (-)-epicatechin contents, the avarage are 3.81 mg/g in cocoa powder, 2.53 mg/g in cocoa liquor and 4.61 mg/g in cocoa beans. Whereas the avarage for (+)- catechin contents are 4.28 mg/g in cocoa powder, 3.49 mg/g in cocoa liquor and 3.02 mg/g in cocoa beans. Levels of caffein and theobromine in 32 samples of chocolate products averaged 0.62-1.14 mg/g and 0.026-0.153 mg/g respectively. Mean values for theobromine and caffeine contents for chocolate coating were 0.82 and 0.07 mg/g respectively. The chocolate coating made from fat substitute had theobromine and caffeine levels ranged from 0.36 0.70 mg/g and 0.027-0.061 mg/g respectively, with mean values of 0.49 mg theobromine/g and 0.039 mg caffeine/g. In local chocolate, the mean theobromine and caffeine levels respectively were 0.72 mg/g and 0.04mg/g in milk chocolate, and 0.85 mg/g and 0.06 mg/g in dark chocolate. As in imported chocolate, the mean theobromine and caffeine levels respectively were 1.05 mg/g and 0.12 mg/g in dark chocolate; 0.76 mg/g and 0.04 mg/g in milk chocolate; and 0.74 mg/g and 0.03 mg/g in white chocolate. Compared with the local chocolate, imported chocolate has higher level of theobromine and caffeine. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ukm.my/mjas/v7_n2/14Nazaruddin.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
