NDLI: Orthric Rieske dioxygenases for degrading mixtures of 2,4-dinitrotoluene/naphthalene and 2-amino-4,6-dinitrotoluene/4-amino-2,6-dinitrotoluene

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Orthric Rieske dioxygenases for degrading mixtures of 2,4-dinitrotoluene/naphthalene and 2-amino-4,6-dinitrotoluene/4-amino-2,6-dinitrotoluene

Content Provider	Semantic Scholar
Author	Keenan, Brendan G. Wood, Thomas K.
Copyright Year	2006
Abstract	Pollutants are frequently found as mixtures yet it is difficult to engineer enzymes with broad substrate ranges on aromatics. Inspired by the archetypal nitroarene dioxygenase, which shares its electron transport with a salicylate monooxygenase, we have created an innovative and general approach to expand the substrate range of dioxygenase enzymes in a single cell. We have developed here a series of novel, hybrid dioxygenase enzymes that function with a single ferredoxin reductase and ferredoxin that are used to transport two electrons from nicotinamide adenine dinucleotide to the two independent terminal oxygenases. Each independent alpha-oxygenase may then be used simultaneously to create orthric enzymes that degrade mixtures of environmental pollutants. Specifically, we created a hybrid dioxygenase system consisting of naphthalene dioxygenase/dinitrotoluene dioxygenase to simultaneously degrade 2,4-dinitrotoluene and naphthalene (neither enzyme alone had significant activity on both compounds) and dinitrotoluene dioxygenase/nitrobenzene dioxygenase to simultaneously degrade the frequently encountered 2,4,6-trinitrotoluene reduction products 2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene.
Starting Page	827
Ending Page	838
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1007/s00253-006-0538-8
PubMed reference number	16933133
Journal	Medline
Volume Number	73
Alternate Webpage(s)	http://www.che.psu.edu/faculty/wood/group/publications/pdf/orthric_DOs.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s00253-006-0538-8
Journal	Applied Microbiology and Biotechnology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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