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Nicotinamide adenine dinucleotide-dependent formate dehydrogenase from Rhodopseudomonas palustris
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yoch, Duane C. Lindstrom, Eloise |
| Copyright Year | 2004 |
| Abstract | SummaryFormate dehydrogenase in extracts of the facultative phototroph, Rhodopseudomonas palustris was shown to be soluble and NAD-linked. The flavin nucleotides, FMN and FAD, stimulated the rate of NAD reduction about fourfold. Reduction of artificial electron acceptors such as DCPIP and cytochrome c was also stimulated by FMN and FAD. The pH optimum for the reduction of NAD was pH 8.0, in contrast to pH 6.8 for cytochrome c and DCPIP reduction. The apparent Kmfor formate as measured by NAD reduction was 2.6×10-4 M. Although the addition of thiosulfate or yeast extract to the formate medium increased both the growth rate and yield of Rhps. palustris, they had little effect on the activity of formate dehydrogenase. |
| Starting Page | 182 |
| Ending Page | 188 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF00409684 |
| PubMed reference number | 4318273 |
| Journal | Medline |
| Volume Number | 67 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/BF00409684 |
| Alternate Webpage(s) | https://doi.org/10.1007/BF00409684 |
| Journal | Archiv für Mikrobiologie |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
