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Near-infrared-excited confocal Raman spectroscopy advances in vivo diagnosis of cervical precancer
| Content Provider | Semantic Scholar |
|---|---|
| Author | Duraipandian, Shiyamala Zheng, Wei Ng, Joseph Low, Jeffrey J. H. Ilancheran, Arunachalam Huang, Zhiwei |
| Copyright Year | 2013 |
| Abstract | Abstract. Raman spectroscopy is a unique optical technique that can probe the changes of vibrational modes of biomolecules associated with tissue premalignant transformation. This study evaluates the clinical utility of confocal Raman spectroscopy over near-infrared (NIR) autofluorescence (AF) spectroscopy and composite NIR AF/Raman spectroscopy for improving early diagnosis of cervical precancer in vivo at colposcopy. A rapid NIR Raman system coupled with a ball-lens fiber-optic confocal Raman probe was utilized for in vivo NIR AF/Raman spectral measurements of the cervix. A total of 1240 in vivo Raman spectra [normal (n=993), dysplasia (n=247)] were acquired from 84 cervical patients. Principal components analysis (PCA) and linear discriminant analysis (LDA) together with a leave-one-patient-out, cross-validation method were used to extract the diagnostic information associated with distinctive spectroscopic modalities. The diagnostic ability of confocal Raman spectroscopy was evaluated using the PCA-LDA model developed from the significant principal components (PCs) [i.e., PC4, 0.0023%; PC5, 0.00095%; PC8, 0.00022%, (p<0.05)], representing the primary tissue Raman features (e.g., 854, 937, 1095, 1253, 1311, 1445, and 1654  cm−1). Confocal Raman spectroscopy coupled with PCA-LDA modeling yielded the diagnostic accuracy of 84.1% (a sensitivity of 81.0% and a specificity of 87.1%) for in vivo discrimination of dysplastic cervix. The receiver operating characteristic curves further confirmed that the best classification was achieved using confocal Raman spectroscopy compared to the composite NIR AF/Raman spectroscopy or NIR AF spectroscopy alone. This study illustrates that confocal Raman spectroscopy has great potential to improve early diagnosis of cervical precancer in vivo during clinical colposcopy. |
| File Format | PDF HTM / HTML |
| DOI | 10.1117/1.JBO.18.6.067007 |
| PubMed reference number | 23797897 |
| Journal | Medline |
| Volume Number | 18 |
| Alternate Webpage(s) | http://biomedicaloptics.spiedigitallibrary.org/pdfaccess.ashx?url=/data/journals/biomedo/25400/jbo_18_6_067007.pdf |
| Alternate Webpage(s) | https://www.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.18.6.067007 |
| Alternate Webpage(s) | https://doi.org/10.1117/1.JBO.18.6.067007 |
| Journal | Journal of biomedical optics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
